Steps / Activities / Participants
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| Step I: Preliminary studies and researches for identification of fundamental principles for achieving new biosensors useful for nitrates and nitrites monitoring
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| Activity I.1: Studies and analyses required for lab scale technology accomplishment |
| Partner P1(CO): Study regarding possible ways for enzyme covalent immobilization on polymers, useful in nitrate and nitrite monitoring. |
| Partner P2: Study for biosensors producing for nitrate and nitrite monitoring. |
Partner P3: Study regarding analytical methods for monitoring nitrates and nitrites existing in water for human consumption.
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| Step II: Researches for elaboration of the biosensors manufacture technologies, based on enzyme covalent immobilization upon polymers
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| Activity II.1: Development of an experimental model- technology for manufacture biosensors useful for monitoring of the nitrates and nitrites content in water |
| Partner P1(CO): Researches for elaboration lab scale technology for achieving enzyme immobilization on polymer membrane. |
| Partner P2: Researches for estimation of enzymatic activity of peculiar membranes holding covalent immobilized enzymes and also for biosensors making. |
| Partner P3: Comprehensive analysis of waters for human consumption emphasizing their nitrates and nitrites content. |
| Partner P4: Morpho-structural analysis of the involved polymers and of the enzymatic nanocomposites. |
| Partner P5: Molecular weight, physical and chemical analysis of polymers, biopolymers and containing enzymes nanocomposite membranes. |
| Activity II.2: Experimental model designing |
| Partner P1(CO): Designs of technological and outfit flow charts in order to perform these new materials. |
Partner P2: Participates at designing activity of technological and outfit flow charts in order to perform these new materials.
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| Step III: Experimental model accomplishment: lab technology for producing enzymatic biosensors useful for monitoring nitrates and nitrites existing in human consumption water |
| Activity III.1: Accomplishment of experimental model - lab scale technology |
| Partner P1(CO): Accomplishment of the producing procedure for polymer membrane holding covalently immobilized enzymes. |
| Partner P2: Accomplishment lab scale procedure for producing biosensors useful for monitoring of nitrate and nitrite levels in waters. |
| Partner P4: Structure characterization of polymer-enzyme composites. |
| Partner P5: Physical and chemical characterization of polymer nanocomposites. |
| Activity III.2: Elaboration of the documentation for functional model - lab scale technology |
| Partner P1(CO): Technology achievement for producing biosensors useful for monitoring the water nitrates and nitrites contents. |
| Partner P2: Participates at technology achievement for producing biosensors useful for monitoring the water nitrates and nitrites contents. |
| Activity III.3: Experimentation of the proposed solution for enzymatic biosensors producing and their utilization in order to monitor the nitrate and nitrite content in water for human consumption |
| Partner P1(CO): Experimentation of the proposed solution for producing membranes containing covalently immobilized enzymes. |
| Partner P2: Experimentation of the proposed solution for producing biosensors useful to monitor the nitrate and nitrite content. |
Partner P3: Experimentation of utilization of biosensors in monitoring of the nitrate and nitrite content from waters for human consumption.
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| Step IV: Demonstration of functionality and utility of resulted technology for enzymatic biosensors realization useful for monitoring nitrates and nitrites content existing in waters for human consumption, patent protection and results dissemination
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| Activity IV.1: Demonstration of functionality and utility of proposed technology, based on covalently immobilization of enzymes on polymers, for biosensors realization |
| Partner P1(CO): Researches for proving technology reproducibility of enzyme covalently immobilization on polymer membrane. |
| Partner P2: Researches for proving reproducibility of technology for producing the new biosensors. |
| Partner P4: Helps the demonstration with peculiar analysis. |
| Partner P5: Complex characterization of polymers and polymer enzymatic composites. |
| Activity IV.2: Technology experimentation and verification |
| Partner P3: Researches for functionality and utility demonstration of enzymatic biosensors for human consumption waters monitoring, comparatively with other methods. |
| Activity IV.3: Designing and elaboration of technical-economical documentation |
| Partner P1(CO): Coordinates and participates at activity of feasibility study elaboration. |
| Partner P2: Participates at feasibility study elaboration. |
| Partner P3: Participates at feasibility study elaboration. |
| Activity IV.4: Elaboration of technical specification |
| Partner P1(CO): Coordinates and participates at activity of elaboration of technical specification. |
| Partner P2: Participates at elaboration of technical specification. |
| Partner P3: Participates at elaboration of technical specification. |
| Partner P4: Participates at elaboration of technical specification. |
| Partner P5: Participates at elaboration of technical specification. |
| Activity IV.5: Large scale dissemination of the results by their communication and national and international publishing |
| Partner P1(CO): Coordinates the whole activity during project progress in order to submit at least 4 scientific communications at international conferences, at least 4 articles (in ISI quoted journals), to edit CD-ROM, web page and to take part at workshops, fairs and exhibitions. |
| Partner P2: Support the dissemination. |
| Partner P3: Support the dissemination. |
| Partner P4: Support the dissemination. |
| Partner P5: Support the dissemination. |
| Activity IV.6: Identification and assigning the results intellectual property right |
| Partner P1(CO): Coordinates the activity of registering at least one patent request. |
| Partner P2: Takes part at patent elaboration activity. |
| Partner P3: Takes part at patent elaboration activity. |
| Partner P4: Takes part at patent elaboration activity. |
| Partner P5: Takes part at patent elaboration activity. |
